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. Author manuscript; available in PMC: 2013 Apr 7.
Published in final edited form as: Arch Neurol. 2009 Feb;66(2):190–199. doi: 10.1001/archneurol.2008.565

Fig. 1. Detection of low molecular weight oligomeric Aβ(di-Cys synthetic dimer) by ELISA.

Fig. 1

A) SEC fraction of dAβ and synthetic monomeric Aβ were visualized on the same SDS eletrophoresis gel by silver staining. Based on the absolute amount of synthetic Aβ peptide loaded on the same gel, the concentration of dAβ was calculated. B) In the presence of β-mercaptoethanol (βME), dAβ was dissociated into monomeric Aβ. C, D) Serial dilutions of dAβ treated +/− βME were analyzed by ELISA using antibody 82E1 (C) or 3D6 (D). Bars, standard errors of means.