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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Immunol. 2012 Dec 19;190(3):970–976. doi: 10.4049/jimmunol.1202805

Figure 2. Kaede-red CD4+ T cells residing in skin are CCR7− CD103+ CD69+.

Figure 2

Flow cytometric analysis of CCR7, CD103 and CD69 expression by Kaede-green CD3+ CD4+ T cells (green solid line) and Kaede-red CD3+ CD4+ T cells (red solid line) remaining within the abdominal skin (a) or migrated from skin to draining LN (b) 24 h after abdominal skin photoconversion. Dashed lines are control “fluorescence minus one” stains of Kaede-green- and Kaede-red CD3+ CD4+ T cells. Graphs depict the average percent of Kaede-green (green bars) and Kaede-red (red bars) CD3+ CD4+ T cells in the skin or draining LN that express CCR7, CD103 or CD69. For CCR7 expression in the draining LN, striped bars depict the percent of CD3+ CD4+ T cells that express intermediate CCR7 and solid bars show the percent of CD3+ CD4+ T cells that express high CCR7. Data are representative of 3 experiments with 9 mice total.