Table 1.
Reagent | Quantity (g) |
---|---|
NaCl | 2.7015 |
NaHCO3 | 0.252 |
Na2CO3 | 0.213 |
KCl | 0.1125 |
K2HPO4 | 0.115 |
MgCl2·6H2O | 0.1555 |
HEPESa | 8.946b |
CaCl2 | 0.1465 |
Na2SO4 | 0.036 |
1.0 M NaOH | c |
2-(4-(2-hydroxyethyl)-1-piperazinyl)ethanesulfonic acid.
HEPES was dissolved in 50 mL of 0.2 M NaOH before addition
Added until pH 7.4.