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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Biomed Mater Res A. 2012 Nov 14;101(6):1571–1581. doi: 10.1002/jbm.a.34462

Table 1.

Preparation conditions for 500 mL of SBF using Oyane et al.'s recipe for m-SBF.27

Reagent Quantity (g)
NaCl 2.7015
NaHCO3 0.252
Na2CO3 0.213
KCl 0.1125
K2HPO4 0.115
MgCl2·6H2O 0.1555
HEPESa 8.946b
CaCl2 0.1465
Na2SO4 0.036
1.0 M NaOH c
a

2-(4-(2-hydroxyethyl)-1-piperazinyl)ethanesulfonic acid.

b

HEPES was dissolved in 50 mL of 0.2 M NaOH before addition

c

Added until pH 7.4.