Table I.
Nanolitre real-time RT-PCR panel target pathogens
| Bacteria | Viruses | Parasites |
|---|---|---|
| Enterohemorrhagic Escherichia coli (EHEC) | Norovirus group 1 (20) | Giardia lamblia (25) |
| stx Ia (9) | ||
| stx IIa (10) | ||
| E. coli O157 (11) | ||
| Salmonella spp. (12) | Norovirus group 2 (20) | Cryptosporidium spp. (26) |
| Shigella spp. (13) | Rotaviruses (21) | |
| Campylobacter spp. (14) | Astroviruses (22) | |
| Yersinia entercolitica (15) | Adenoviruses 40/41 (23) | |
| Clostridium difficile (16) | Sapoviruses (24) | |
| Clostridium difficile tcd Bb (17) | ||
| Listeria monocytogenes (18) | ||
| Vibrio parahaemolyticus (19) |
Number in brackets indicates number in reference list for publication describing assay.
a
stx I and stx II are genes encoding for Shiga toxin 1 and 2, one of which is generally present in all strains of EHEC.
b
tcd B is the gene encoding for C. difficile toxin B, the key virulence factor.