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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Feb 11;432(3):466–471. doi: 10.1016/j.bbrc.2013.01.121

Figure 1. Timecourse of Akt and S6K1 phosphorylation response to low OT.

Figure 1

Caco2BB cultures were stimulated with low OT (7.8 nM) in fresh growth medium (FGM) and FGM alone (0). Electrophoretic bands were developed with anti-pAktT308, with anti pAkt S473 and anti-total Akt antibodies. Note that low OT downregulates pAktT308, the maximal effect occurring at 30 min. FGM also has a time-dependent effect on pAktT308. Different time dependence is seen for pAktS473. The phosphorylation of S6K1 observed after FGM was attenuated by OT.