Figure 1.

Impact of dendritic cells (DC) on tumour growth and immune reconstitution. (A) The experimental sequence includes subcutaneous implantation of 10⁶ Neuro-2a cells (day −5), followed by radiation at 700rad and transplantation of 7 × 10⁶ allogeneic BMC (H2K^b→H2K^a). 10⁶ donor DC (H2K^b) were infused at 7 days post transplantation. (B) Tumour size in allografted mice bearing tumours (n=21) and immunised with tumour-pulsed donor DC (DC^neuro2a, n=14). (C) Lysis of Neuro-2a cells in vitro as determined from LDH release by effector splenocytes from allografted mice (allo-BMT) and with DC^neuro2a immunisation (BMT+DC) at various target:effector ratios. Data represent five mice in each group from one of the repeated experiments.