Table 3. Frequency of NY-ESO-1 antibody positives in gastric cancer patients in whom the NY-ESO-1 antigen was or was not detected by IHC or RT–PCR.

	IHC
	Positive	Negative	Total
mRNA
Positive	5/6 (83.3)	0/0 (0.0)	5/6 (83.3)
Negative	3/13 (23.1)	1/41 (2.4)	4/54 (7.4)
Total	8/19 (42.1)	1/41 (2.4)	9/60 (15.0)

Abbreviations: IHC=immunohistochemistry; RT–PCR=reverse transcription–polymerase chain reaction.

Frozen and formalin-fixed tissue specimens from 60 patients, including 12 stage I, 12 stage II, 20 stage III, and 16 stage IV patients, were analysed.

All stage IV patients had previously undergone surgical treatment.

Values within parentheses are percentages.