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. 2013 Apr 5;9(4):331–342. doi: 10.7150/ijbs.6022

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Influence of 20E on expression of BdCHS1 and its two alternative splicing exons. The insects were injected with 20-hydroxyecdysone (20E) at day-2 of third-instar larvae, and five individuals each were analyzed 1, 4, 8, and 12 h after treatment using qPCR. α-Tubulin was used as an internal reference gene. Data are means ± SE of three biological replications. (A) BdCHS1; (B) BdCHS1a; (C) BdCHS1b. CK: control insects; 20E: insects injected with 20E. Significantly differences between treatment and control are indicated with * (P < 0.05) or ** (P < 0.01, t test).