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. 2013 Mar 16;9(3):303–312. doi: 10.7150/ijbs.6214

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 1 — The stability and reproducibility of multiplex-RT qPCR. (A, B) Pearson correlation of U6B-normalized miR-26a (A) and miR-26b (B) readings from multiplex- and singleplex-RT qPCR in cohort 1 (n=129). (C, D) Pearson correlation of six individual reference-normalized miR-26 readings derived from SBC and NCI. The multiplex-RT qPCR assay was performed in the same RNA isolates (n=10) (C) or different RNA isolates from two different tumor regions of the same FFPE specimens (n=10) (D). (E, F) Forest plots showing hazard ratios in log2 scale for the overall survival of HCC patients from cohort 1 treated with IFNα versus controls without or with selection by U6B-normalized miR-26a (E) or miR-26b (F). The median of U6B-normalized miR-26a and miR-26b were used as cut-off points.