Fig 2.

Effects of Wnt5a on the melanogenesis of melan-a cells. (A) Tyrosinase activity of melan-a cells infected with different doses of AdWnt5a or AdGFP analyzed by tyrosinase activity assay. (B) Melanin synthesis of melan-a cells infected with AdWnt5a or AdGFP. (C-D) Effect of Wnt5a on the expression of TRP1 and tyrosinase in melan-a cells. (C) Melan-a cells were infected with AdWnt5a or AdGFP. RT-PCR analyses were performed with primers specific for TRP1, tyrosinase and GADPH after 0h, 16h, 24h and 48h. (D) Melan-a cells were infected with AdWnt5a or AdGFP. After 48 h, Western blot analyses were performed with antibodies specific for TRP1, tyrosinase and GADPH. (E) Melan-a cells infected with AdWnt5a or AdGFP were implanted subcutaneously into nude mice. Visual pigmentation were exhibited in Day 1 and 3. Implanted sites were retrieved and subjected to Fontana-Masson staining. Melanin-positive cells were indicated with arrows. Bar=50 μm. These data were representative results of three independent experiments. *p< 0.05, **p< 0.01.