FIG. 8.

iNOS mRNA silencing by cSCK•siRNA•GALA nanocomplex in RAW264.7 cells. (A) cytoxicity of PAEA₁₆₀-b-PS₃₀ cSCK-pa₁₀₀ after 24 h in RAW cells that had been induced for 24 hours with LPS/γ-IFN as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (B) Cytotoxicity of cSCK•GALA complexes in induced RAW cells in the absence or presence of different siRNA concentrations as determined by the MTT assay. (C) Knockdown efficiency in RAW cells with cSCK (10 μg/mL)•siRNA (50 nM)•GALA (0, 0.1, 0.2, 0.3 μM) nanocomplexes in induced RAW cells. iNOS mRNA level was measured by real-time polymerase chain reaction after 8 hours. The P value for the observed difference in expression with and without GALA was <0.01.