Table 2.
Five Identified miRNAs Associated with Their Experimentally Validated Targets
| miRBase ID | Mature miRNA | Validated miRNA targetsa | Gene silenced in screen | Sequence (5'→3') |
|---|---|---|---|---|
| MIMAT0004814 | hsa-miR-654-3p | 1 gene (CDKN1A) | 4 genes (CDC34, RAMP3, IL4, SEMA6D) | UAUGUCUGCUGACCAUCACCUU |
| MIMAT0003320 | hsa-miR-650 | 1 gene (ING4) | 2 genes (LYRM1, DSCAM) | AGGAGGCAGCGCUCUCAGGAC |
| MIMAT0004487 | hsa-let-7f-2-3p | 21 genes (ASS1, CCND1, CDKNIA, CSN1, CSN6, CSN8, EEFIA2, ESM1, FDPS, IL-13, KIF1A, KLK10, KLK6, MYC, NIS, NTS, PRDM1, TFF1, TG, TITF1, VIM) | 4 genes (LILRA2, IGSF10, I L1RAP, OR2T27) | CUAUACAGUCUACUGUCUUUCC |
| MIMAT0000275 | hsa-miR-218-5p | 63 genes (ACO26713.5, ACTN1, AGPAT5, AHI1, AKAP8, ATP6V0E, BIRC6, BLCAP, BTBD7, BTF3L4, C130RF7, C5ORF5, CAMTAI, CDKNIB, CENPO, CNTNAP2, COL1A1, COL4A1, DUSP18, EBP, EFNA1, EFNB2, ETS2, FOXN2, GLCE, GPM6A, HECTD2, IKBKB, KATNALI, KIAA0391, KIAA1462, KLF9, KLHL13, LAMBS, LASP1, MAFG, MBNL2, MRPS27, NACC1, NFE2L1, NUP93, OLIG2, PHC3, POGK, POZ, PTPN11, PUM2, RARA, RHBDD1, RITZ, RNF38, SERINC3, SFRS12, SLC11A2, SLC38A1, SP1, SSH1, STAM2, THOC2, TNIP2, TPD52, TTC33, VOPP1) | 5 genes (SOX30, IL12RB2, IL18BP, MY018A, CDK10) | UUGUGCUUGAUCUAACCAUGU |
| MIMAT0000437 | hsa-miR-145 | 88 genes (ACBD3, AKR1B10, ALDH3A1, ALPPL2, AP1G1, APHIA, ARL6IP5, BNIP3, C110RF58, C110RF65, C3ORF34, C6ORF115, CBFB, CCDC25, CCDC43, CCNA2, CDKNIA, CIQTNF5, CLINTI, DDR1, DFFA DTD1, ELK-1, ERLINI, F11R, FAM108C1, FAM3C, FAM45A, FAM79B, FBXO28, FLI1, FLJ21308, FSCN1, GMFB, GOLPH2, HLTF, HOXA9, IFNBI, IGFIR, IGF-IR, IRSI, IRS-I, JAM-A, KLF4, KLF5, KREMENI, KRT7, LOC203547, LOC340888, LOC342705, LOC400011, LYPLA2, MAP2K6, MEST, MIXL1, MMP1, MMP14, MTMR14, MUC1, MYC, MYO6, NDRG2, NDUFA4, NHEDCI, NIPSNAPI, OCT4, PARP8, PHF17, PIGF, PODXL, POU5F1, PPP3CA, PTP4A2, RASA1, ROBO2, RTKN, S0CS7, SERINC5, SOX2, STAT1, SWAP70, TIRAP, TMEM9BMMP12, TMOD3, TPM3, TSPAN6, USP46, YES1) | 5 genes (RABI3, KISS1, LCP1, ENC1, KDELR1) | GUCCAGUUUUCCCAGGAAUCCCU |
Seed heptamer sequences within the mature miRNA sequence are presented in boldface.
a
Information on experimentally validated targets was obtained from Tarbase 6.0.20