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. 2013 Apr;11(3):191–205. doi: 10.1089/adt.2012.477

Fig. 2.

Fig. 2.

Validation results and expression analysis with quantitative polymerase chain reaction (qPCR). Cells were tested against a focused panel of siRNA duplexes using reverse-transfection, then evaluated with image analysis and qPCR. (A) Representative images of cells treated with siRNA duplexes for EIF2C2, DGCR8, DROSHA, DICER1, XPO5, and PLK1. Images were acquired using the IN CELL Analyzer 3000 (INCA3000) with green channel for EGFP signal and blue channel for Hoechst-stained nuclei. (B) Gene expression analysis of cells treated with 5 nM of siRNA for 2 days. (C) miRNA expression analysis of cells treated with 50 nM of siRNA for 6 days.