Table 2.
Validation of Multiplexed-Assay
| Gene | EGFP gaina | Cell viabilityb | Gene knockdownc | Reference |
|---|---|---|---|---|
| EIF2C2 (Ago2) | 2/3 siRNA | No effect | 2/3 siRNA | 12 |
| DGCR8 | 3/3 siRNA | 1/3 siRNA toxic | 1/3 siRNA | 9 |
| DROSHA (RNASEN) | 2/3 siRNA | 1/3 siRNA toxic | 3/3 siRNA | 7,8 |
| TNRC6B | 1/3 siRNA | No effect | 3/3 siRNA | 31 |
| DICER1 | 2/3 siRNA | 2/3 siRNA toxic | 3/3 siRNA | 11 |
| TARBP2 | 1/3 siRNA | 1/3 siRNA toxic | 3/3 siRNA | 30 |
| EIF2C1 (Ago1) | No gain | 1/3 siRNA toxic | 3/3 siRNA | 28 |
| EIF2C3 (Ago3) | 2/3 siRNA | 1/3 siRNA toxic | 0/3 siRNA | 28 |
| EIF2C4 (Ago4) | No gain | No effect | 2/3 siRNA | 28 |
| TNRC6A | No gain | No effect | 2/3 siRNA | 31 |
| TNRC6C | No gain | No effect | 2/3 siRNA | 31 |
| DHX9 | No gain | 1/3 siRNA toxic | 3/3 siRNA | 27 |
| XPO5 | No gain | No effect | 3/3 siRNA | 10 |
| MOV10 | 1/3 siRNA | 1/3 siRNA toxic | 3/3 siRNA | 29 |
| SCPEP1 | No gain | 1/3 siRNA toxic | 3/3 siRNA | 32 |
| KIF11 | No gain | 3/3 siRNA toxic | 3/3 siRNA | 33 |
| PLK1 | 1/3 siRNA | 3/3 siRNA toxic | 3/3 siRNA | 34 |
a
Number of siRNA for given gene resulting in >2σ EGFP fluorescence above background as determined by Silencer Select Negative Control #1 siRNA.
b
Number of siRNA resulting in cytotoxicity with >80% inhibition relative to data median.
c
Number of siRNA resulting in knockdown using a threshold of 40% gene expression remaining.