Figure 5.

The anti-apoptotic effects of Grail. (a) C2C12- and C2C12-Grail stable cell lines were treated with DMSO (control), Act D (30 nM), or UV (200 J/m²) for 48 h. The cells were later harvested and cell cycle profiles were analyzed by FACS. (b) Saos-2 cells were infected with adenoviral p53 and/or Grail for 48 h. The cells were harvested and the cell cycle profiles were analyzed by FACS. (c) C2C12/shp53- and C2C12/shp53/Grail-stable cell lines were treated with DMSO (control), Act D (30 nM), or UV (200 J/m²) for 48 h. The cells were harvested, and the cell cycle profiles were analyzed by FACS. (d) Saos-2 cells (with or without Grail expression) were treated with UV (200 J/m²) for 48 h as indicated. Cells were subsequently harvested, and the cell cycle profiles were analyzed by FACS