Figure 7.

The p53/Grail negative feedback loop. (a and b) H1299 cells were infected with Ad-p53 expression vector. At indicated time, cells were harvested to analyze the cell cycle profiles by FACS (a), and analyze the mRNA amount by quantitative real-time PCR (b). (c and d) U2OS cells stably expressing the indicated shRNAs were treated with DMSO (control) or Act D (20 nM) for 24 h. Representative immunofluorescence images showing triple labeling for Grail (green), p53 (red), DAPI (blue), and the merged images of Grail/p53 and Grail/p53/DAPI