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. 2013 Mar 18;110(14):5582–5587. doi: 10.1073/pnas.1215438110

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Fig. 4. — Changes in in vitro colony formation and IL-3 receptor expression but no effect on CFU-S formation, sensitivity to γ irradiation, and the pool size of mature myeloid populations. (A) Plots show in vitro colony formation after culture of wild-type or CD133 KO bone marrow cells with GM-CSF, IL-3 plus erythropoietin (Epo), G-CSF plus M-CSF plus IL-3 with and without SCF, IL-3, or Epo (from left to right). Plots show means ± SD of nine mice per genotype (GM-CSF, IL-3+Epo), six mice per genotype (Epo), and five mice per genotype (G-CSF+M-CSF+IL-3± SCF, IL-3). Significant differences were indicated. *P = 0.05–0.01; **P = 0.01–0.001. (B) Plot shows frequency of CD123^hi cells in Lin⁻ Sca-1⁻ bone marrow cells with or without in vivo stimulation with IL-3 complexes (IL-3C). Results show means ± SD of 11 or 5 mice per genotype for PBS control or IL-3 complex injections, respectively. (C) Plot shows the mean fluorescence intensity (MFI) of CD123 expression on CD123^hi cells. Data presentation as described in B. (D) Macroscopically visible colonies in the spleen of recipient mice were counted 8 d [(CFU-S day 8 (CFU-S_d8)] after transplantation of CD133 KO or wild-type bone marrow (BM) cells. Photographs show one representative recipient spleen from each donor. (E) Survival curve of wild-type (solid line) and CD133 KO (broken line) mice after lethal irradiation. Data are pooled from three independent experiments using a total of 25 wild-type or CD133 KO mice. (F) Dot plots show the expression of CD11b vs. Gr-1, PDCA1 vs. CD11c, MHCII vs. CD11c, CD11b vs. F4/80, and side scatter (SSC) vs. forward scatter (FCS) (from left to right) on spleen cells from wild-type (Upper) and CD133 KO (Lower) mice on CD3-, B220-, and Ter119-negative cells. Arrows indicate the population that was further resolved in the following dot plot. cDC, conventional dendritic cells; Eo, eosinophils; Mono/Mp, monocytes/macrophages; pDC, plasmacytoid dendritic cells; PMN, neutrophils; RP-Mp, red pulp macrophages. Data are summarized in E. (G) Graphs show the cell number of spleen cells, PMNs, cDCs, pDCs, RP-Mps, eosinophils, and monocytes/macrophages (from left to right) in wild-type (closed bar) and CD133 KO (open bar) mice identified as shown in C. Means ± SD are given (n = 6 mice per genotype).