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Immunostaining for ET-1. Representative photograph a chronic active MS plaque (darker area) and surrounding NAWM (lighter area) immunostained for ET-1 by using the 3′3-diaminobenzidine tetrahydrochloride method. Blood vessel walls in the plaque and NAWM (red arrows) were positive for ET-1. Reactive astrocytes in the plaque (black arrows) were ET-1–positive, whereas astrocytes in NAWM were ET-1–negative (magnification of 2×).
