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. 2013 Mar 18;110(14):5486–5491. doi: 10.1073/pnas.1220306110

Fig. 2.

Fig. 2.

The HORMA domain of Atg13 is necessary for the recruitment of downstream factors in autophagy. (A) Representative microscopy images of atg11Δ atg13Δ ATG14-GFP cells transformed with vector, ATG13, and ATG13ΔHORMA and treated with rapamycin. (B) Quantification of the Atg14 puncta from A using a brightness threshold of fourfold above the average background fluorescence. A total of three trials with 100 cells counted per trial were made. (C) Representative microscopy images of atg11Δ atg13Δ GFP-ATG8 cells transformed with vector, ATG13, and ATG13ΔHORMA and treated with rapamycin. (D) Quantification of the Atg8 puncta from C. A total of three trials with 100 cells counted per trial. Error bars in B and D represent the SD of triplicate experiments.