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Csy4 H29A is a conditional endoribonuclease. (A) Crystal structure of WT Csy4 bound to its RNA substrate (Protein Data Bank ID code 4AL5) (Left) and close-up of the Csy4 active site (Right). The active site histidine (H29; shown in red) serves as the general base for cleavage of the phosphate backbone at the base of the RNA hairpin. (B) In vitro cleavage activity of WT and mutant Csy4. Cleavage products were separated by 12% denaturing PAGE and visualized with ethidium bromide staining.
