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Performance of a two-layer linear CHA cascade composed of chemically synthesized hairpins from different purification processes. (A) Experiments with D-pure hairpins. (B and C) Experiments with DNB-pure M1 and DN-pure A1, M2, and A2. (A–C, Bottom) The combinations of reaction components are shown. A and B focus on the dissection of circuit leakage by incrementally adding reactants. C shows the response of the two-layer cascade to different concentrations of the initial trigger (C0).
