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. 2013 Mar 18;110(14):5516–5521. doi: 10.1073/pnas.1216596110

Fig. 4.

Fig. 4.

SET7/9-mediated SUV39H1 methylation induces heterochromatin relaxation in response to DNA damage. (A) H1299 cells were treated with 1 μM Adr for the indicated times. The relative expression levels of Sat2 and α-Sat were measured by real-time PCR. (B) H1299 cells were treated with 1 μM Adr for 24 h. The relative occupancy of H3K9me1, H3K9me2, and H3K9me3 within the region of Sat2 and α-Sat was analyzed by real-time PCR. (C) A si-RNA control (si-Ctr) or SET7/9 siRNA fragment was transfected into H1299 cells for 48 h with or without 1 μM Adr for the last 24 h. The relative expression of Sat2 or α-Sat was measured by real-time PCR. (D) H1299 cells were treated as in C, and the enrichment of H3K9me3 in the region of Sat2 or α-Sat was analyzed by real-time PCR. (E) A fragment of siRNA against SET7/9 or SUV39H1 was transfected into H1299 cells, alone or together, for 48 h, with 1 μM Adr for the last 24 h. The relative expression of Sat2 or α-Sat was measured by real-time PCR. (F) H1299 cells were treated as in E, and the enrichment of H3K9me3 within the region of Sat2 or α-Sat was measured by real-time PCR. *P < 0.05; **P < 0.01; NS, no significant difference.