Fig. 4.

Naïve CD8⁺ T-cell precursor frequency is lower for the NPM6I and NPM6T escape variants. Naïve D^bNP₃₆₆⁺ and D^bNPM6X⁺CD8⁺ precursors within macroscopic lymph nodes and spleens were identified with the D^bNP₃₆₆-PE and D^bNPM6X-PE tetramers (28). Data represent the mean (per group) for three to seven B6 [H-2^b] mice (black circles), BalbC [H-2^d] mice (open squares), and TCR-transgenic OT-I [H-2^b] mice (open circles). *P ≤ 0.01; #P < 0.05; **P < 0.05 relative to D^bNP₃₆₆⁺CD8⁺ precursors. Experiments were repeated two to four times.