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. 2013 Mar 4;110(14):5588–5593. doi: 10.1073/pnas.1301019110

Fig. 5.

Fig. 5.

Gene expression profile in human fibroblasts transduced with 5F. Gene expression profile was analyzed by microarray or quantitative PCR (qPCR) in HFFs or AHCFs 4 wk after transduction with 5F. (A) Heat map of microarray data illustrating differentially expressed 2,436 genes in HFFs, 5F-transduced HFFs, and adult human heart. Red indicates up-regulated genes; green indicates down-regulated genes. (B) Heat map of selected genes. Genes that encode cardiac contractile proteins, cardiac peptides, calcium handling genes, cardiac transcription factors, and genes involved in cardiac metabolism were up-regulated. In contrast, genes encoding nonmyocyte markers were down-regulated. (C) Gene expression analyses by qPCR in HFF and AHCFs transduced with 5F. ACTC1, TNNT2, MYL7, MYH6, and TPM1 are sarcomere genes; ATP2A2, GJA1, and GJA5 are cardiac channel genes; NPPA and NPPB are cardiac peptide genes; COL1A2, COL3A1, and S100A4 are nonmyocyte genes. Expression of cardiac and nonmyocyte genes was quantified by qPCR. UD, undetectable.