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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Cell Metab. 2013 Mar 5;17(3):411–422. doi: 10.1016/j.cmet.2013.02.009

Fig. 3. Adipocyte MHCII and ARTs changes occur early after HFD, while ATM changes occur later.

Fig. 3

RT-PCR analysis of A) adipose tissue, B) CD45-depleted adipocytes, C) ARTs and D) ATMs of male C57BL/6 mice fed HFD for 1, 2, 3, 4 or 12 weeks; and E) adipose tissue, F) CD45-depleted adipocytes, G) ARTs and H) ATMs of similar weight male ob/ob mice and 12wk HFD-fed C57BL/6 mice. (N=4-8/group, p<0.05 and >1.5-fold absolute change vs. matching chow control (*) or ob/ob mouse (†) expression by Mann-Whitney tests.) ART and ATM samples represent pooled material obtained from 4 (adipocytes) or 8 (ARTs and ATMs) mice. All data are normalized against the expression of matching chow-fed lean control mice (red line). RT-PCR analysis of human SVF expression of I) IFN and J) CD3-normalized expression of the ART lineage markers TBX21, FOXP3 and GATA3 (N=6-15, *p<0.05 by Welch’s T-test.) Data represent Means±SEM.