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Interaction of TRPC1 with β-tubulin. A: Yeast two-hybrid interactions between the N (aa 1-349) or C-terminus (aa 643-793) of TRPC1 (NTRPC1 and CTRPC1) and β-tubulin protein. Quantitative β-gal assay were 18 and 25 for NTRPC1/tubulin and for the positive controls, 0 indicates no β-gal activity. B: Proteins identified by mass spectrometer, TRPC1-specific immunoprecipitate was trypsined and individual peptides were sequenced and matched against the protein data bank.
