Fig. 4.

Colchicine treatment decrease thapsigargin stimulated TRPC1 activity. Ca²⁺ influx were measured in Tg-stimulated cells in a Ca²⁺-free buffer, followed by addition of 1 mM Ca²⁺ to the medium. A, C: Fluorescence traces in either control ARPE cells (A) or colchine-treated ARPE cells (B) stimulated with Tg. B, D: Bar graphs showing the relative Ca²⁺ influx in the absence and in the presence of extracellular Ca²⁺. “*” indicate values that are significantly different from that of the respective control condition (P < 0.02, number of cells is indicated in each case). Internal Ca²⁺ release and basal Ca²⁺ influx were not altered by colchine treatment.