Fig. 1. TRP channel transcriptome in SH-SY5Y cells and expression of TRPC1 in the presence of MPP⁺.

A, RT-PCR on SH-SY5Y cells using trpc-, trpv-, and trpm-specific primers. B, Western blot on crude membranes prepared from SH-SY5Y cells treated with MPP⁺ (250 μm) in a time-dependent manner (0, 1, 3, 6, 10, and 24 h). The upper blot was probed using anti-TRPC1 antibody, and the lower blot was probed with anti-SERCA2B antibodies.