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. Author manuscript; available in PMC: 2013 Apr 8.
Published in final edited form as: J Biol Chem. 2004 Nov 12;280(3):2132–2140. doi: 10.1074/jbc.M407384200

Fig. 2. MPP+ induces TRPC1 translocation.

Fig. 2

A, localization of endogenous TRPC1 protein in SH-SY5Y (control) shows no treatment, (+ MPP+) shows cells treated with 250 μm MPP+ for 10 h. Anti-TRPC1 antibody and rhodamine-conjugated secondary antibody were used to detect endogenous TRPC1 protein. B, localization of the TRPC1 protein in cells either overexpressing TRPC1 or in TRPC1 down-regulated cells subjected to MPP+ treatment for 10 h. C, detection of TRPC1 protein in SH-SY5Y cell membrane. Lane 1, control; lane 2, cells transfected with antisense htrpc1; lane 3 cells overexpressing TRPC1.

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