Fig. 4. Activation of TRPC1 increases SH-SY5Y protection from MPP⁺.

Shown is a representative bar graph with the percentage of cell survival as detected via MTT assay. Control cells, TRPC1 expressing cells as Ad-TRPC1 (TRPC1), or as antisense (AS) were treated with MPP⁺. These data are the averages of three independent experiments performed in triplicate. *, values significantly different (p < 0.05) from control value without any treatment. **, values significantly different (p < 0.05) from all sets of experiments. The cells were transiently transfected (for 24 h) with Ad-TRPC1 encoding virus or trpc1 antisense cDNA. Other details of the experiment are provided under “Experimental Procedures.” Carbachol (CCh), thapsigargin (Tg), La³⁺, and 2APB were added 10 min prior to the addition of MPP⁺.