Effect of hypertonic salts on regional levels of mRNAs encoding inhibitors of inflammation. (A) Location of salt application, laser-Doppler flowmetry, and brain sectioning into frontal (Fr), parietal (Pa), and occipital (Oc) samples for RNA extraction. (B) Timecourse of experimental protocol and animal sacrifice for tissue sampling. (C) Northern blots of representative animals sacrificed at various times after KCl application. For each animal, samples from the hemisphere ipsilateral to the application site are indicated by the filled triangles; samples from the contralateral hemisphere are indicated by the open triangles. Control is an unoperated animal. (D) Northern blots of representative animals sacrificed at various times after application of NaCl.