Table 1.

Hydrolysis of αA-61-70 and αA-76-85 peptides by bovine lens protease extracts.

Peptide	Incubation time (h)	% Peptide hydrolyzed
αA-61-70	0	0
	4	10.0
	8	26.7
	16	26.8
αA-76-85	0	0
	4	97.8
	8	100

Synthetic peptides were incubated with bovine lens extract at 37°C for different time intervals. The hydrolyzed peptides obtained after 10 kDa centrifuge filtration were purified using RP-HPLC using C18 analytical column. The amount of substrate hydrolyzed was calculated from peptide peak area. αA-76-85 peptide was completely hydrolyzed in 4-8 h, whereas αA-61-70 peptide was only partially hydrolyzed even after prolonged incubation. Lens extract incubated without synthetic peptides and analyzed at the same time points did not have any peak matching the synthetic peptides used in this study or the products generated by the action of protease.