. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Exp Eye Res. 2013 Feb 11;109:51–59. doi: 10.1016/j.exer.2013.01.016

Table 2.

Hydrolysis of MCA-62-70 substrate by lens protease in the presence of inhibitors.

Mechanistic type	Inhibitor	Concentration	Relative activity (%)
1. Serine	DFP	1mM	78
1. Serine	3, 4-DCI	500μM	15

2. Cysteine	E-64	1mM	100
	N-methylmaleimide	0.2mM	12
	H₂O₂	0.5mM	48
	DTNB	0.2mM	1

3. Metallo-proteinase	1,10 Phenanthroline	1mM	1
		500μM	8
		100μM	59

	EDTA	5mM	46
	EDTA	10mM	15

	EGTA	1mM	51

4. Others	Bestatin	0.5mM	88
	Iodoacetamide	1mM	97
	DTT	2.5mM	4
	TCEP	1mM	49
	CaCl₂	2mM	94
	ZnCl₂	1mM	72
	MgCl₂	1mM	111

Bovine lens protease-rich fraction (1 mg) was pre-incubated with different reagents at 37 °C for 30 min, and the residual proteolytic activity was measured using MCA-62-70 (5μg) substrate as described under methods.