Table 1.
Analysis of the effect of experimental treatments on larval and juvenile traits by generalized linear (mixed effect) modelling. (Results shown concern reduced models issued from backward elimination. For each trait and covariate, the following elements are provided: the effect estimate α together with its standard error (s.e.) (α) in the reduced model; the χ2 statistic (with 1 d.f. for all tests) and the resulting p-value for the type II test from the complete model. Larval survival probability: generalized linear mixed effect model for Bernoulli data with logit link, and replicate tanks as a random effect; probability of metamorphosis: generalized linear model for binomially distributed data with logit link; post-metamorphic standard length: generalized least-squares model with residual variance differing across T × F levels to account for heteroscedasticity. Juvenile body mass growth and final juvenile body mass: generalized least-squares models with residual variance differing across T × F × L and t × T × F × L combinations to account for heteroscedasticity. For juvenile body mass growth, terms excluding time t measure effects on initial body mass just after the intermediate growing phase, since t was set at 0 on this date, while terms including t evaluate effects on body mass growth rate. α0, intercept; T, larval rearing temperature (continuous); F, larval feeding regime (factor: A/AE); L, juvenile dietary lipid content (continuous); t, time (dph); n.a., not applicable.)
trait | covariate | α | s.e. (α) | ![]() |
p-value |
---|---|---|---|---|---|
larval survival | α0 | −0.141 | 0.180 | n.a. | n.a. |
proportion of metamorphosed individuals | α0 | −24.702 | 3.828 | n.a. | n.a. |
t | 1.034 | 0.160 | 129.763 | <0.001 | |
T | 1.095 | 0.208 | 54.333 | <0.001 | |
F | 1.572 | 0.580 | 8.540 | 0.003 | |
final larval standard length | α0 | 11.114 | 0.184 | n.a. | n.a. |
T | 0.274 | 0.099 | 6.939 | 0.008 | |
juvenile body mass growth | |||||
initial body mass | α0 | 9.993 | 0.082 | n.a. | n.a. |
T | 0.132 | 0.027 | 209.742 | <0.001 | |
F | −1.012 | 0.116 | 3.148 | 0.076 | |
T × F | 0.445 | 0.038 | 125.733 | <0.001 | |
growth rate | t | 0.069 | 0.003 | 540.040 | <0.001 |
t × F | −0.009 | 0.003 | 8.225 | 0.004 | |
t × L | −0.003 | 0 | 59.410 | <0.001 | |
final juvenile body mass | α0 | 14.859 | 0.252 | n.a. | n.a. |
T | 0.109 | 0.081 | 56.591 | <0.001 | |
F | −1.795 | 0.267 | 15.549 | <0.001 | |
L | −0.193 | 0.024 | 59.410 | <0.001 | |
T × F | 0.532 | 0.103 | 23.732 | <0.001 |