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(a) After six months of dietary restriction hepatic Mup5 mRNA levels in male C57BL6 mice decrease compared with ad libitum fed mice. (b) Re-feeding for six months increased Mup5 gene expression compared with AL mice. The Mup5 mRNA to β-actin mRNA ratios for AL- and DR-fed mice as determined by real-time PCR are shown. The ratio for the AL control was set to 1. Data are shown as means +s.e.m, n ≥ 7 per group. *p ≤ 0.05 compared DR with AL.
