Figure 4.

Hepatic and urinary Mup protein levels decrease upon dietary restriction and return to higher than ad libitum fed mice levels after re-feeding (RF). (a,b) Hepatic and (c,d) urinary Mup protein levels in male C57BL6 mice after six months of dietary restriction and six months of RF were detected by Western blotting. (e) A three-week dietary restriction does not affect hepatic Mup levels. Representative Western blots of four mice per group are shown and for the corresponding densitometries of all animals were used. As protein excretion in urine is limited, detecting a loading control in urinary samples was not possible.