Figure 5.

Immunohistochemical detection of a PGP-like transporter and Na⁺K⁺-ATPase on sections of larval lepidopteran ganglia: (a) Frozen section of a D. nerii ganglion treated with the anti-PGP antibody C219. Specific labelling (brown precipitation, black arrow) can be seen in the periphery of the ganglion. Above, treatment; below, control (primary antibody omitted). (b) Paraffin section of a ganglion of a M. sexta caterpillar. Orange (white asterisk): specific label of Na⁺K⁺-ATPase by the antibody α5. Blue (white arrow): nuclei stained with 4′,6-Diamidino-2-phenylindole dihydrochlorid (DAPI). (Online version in colour.)