Table I. Genome-wide methylation sequencing summary for CD4+ T cell DNA cut with HpaII or MspI restriction nuclease.
Cell Type | Nuclease | No. of Hits in Genome | Unique Tagsa | % |
---|---|---|---|---|
Naive | HpaII | 9,902,632 | 5,074,880 | 51 |
MspI | 12,994,381 | 5,499,474 | 42 | |
Effector | HpaII | 9,349,718 | 6,039,406 | 65 |
MspI | 9,673,142 | 6,140,353 | 63 | |
Memory | HpaII | 13,582,273 | 7,055,612 | 52 |
MspI | 9,943,128 | 4,193,004 | 42 | |
Total | 65,445,274 | 34,002,729 | 52 |
Twenty-base pair MSCC tags were mapped in the genome.
Number of tags in restriction sites for analysis of DNA methylation.