Table IV. Summary of CD4⁺ T cell sequencing.

Cell Type	Sequenced Tags	Unique Tags	Mapped Tags (one locus)	Tags in RefSeq	Gene No.	Gene No. (>1 copy)
CD4+ naive T cells	12,088,592	7,883,186	4,122,853	3,382,975	14,064	8,715
CD4+ effector T cells	8,660,468	4,547,959	4,449,231	2,790,122	12,877	8,756
CD4+ memory T cells	11,442,151	6,258,543	3,916,175	3,179,174	13,384	8,138

Unique tags were aligned to a position unambiguously. Unique tags in TSSs were the number of unique tags mapped to regions within 500 bases of the representative TSSs of genes in the RefSeq database. Unique tags were categorized into three groups based on the number of mismatches in individual alignments. Effector T cells were generated from CD4⁺ T cells from DO11.10-Tg mice stimulated with an OVA peptide plus allophycocyanin conditions for 5 d in vitro. Memory CD4 T cells were isolated from spleen and lymph node at 4 wk after cell transfer. 1 copy = 20 tags/3 million tags, because human cells are predicted to contain 300,000 mRNA molecules.