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. Author manuscript; available in PMC: 2013 Apr 8.
Published in final edited form as: Exp Physiol. 2011 Oct 3;97(1):89–101. doi: 10.1113/expphysiol.2011.060673

Figure 2.

Figure 2

(a), RNCM were transduced with four different dose of Ad-CMV-EGFP or Ad-G-AT2R-EGFP (0.5 ifu/cell, 5 ifu/cell, 50 ifu/cell, and 100 ifu/cell) for 48 h. PBS was used as negative control and Ad-CMV-EGFP was used as viral control. (b), Apoptosis was detected using the DeadEnd Colorimetric TUNEL System kit. The brown-colored nuclei as indicated by arrow head represent TUNEL-positive (apoptotic) cells. (c), Representative fluorescence micrographs from RNCM transduced with 50 ifu/cell of Ad-CMV-EGFP or Ad-G-AT2R-EGFP. (d), AT2R mRNA level was quantified by realtime RT-PCR. (P<0.05, * 24h vs 0h and 6h; # 36h vs 0h, 6h, 12h, and 72h; $ 48h vs 0h,6h,12h,24h,60h, and 72h;** 60h vs72h.). Scale bars, 50 µm.