Fig. 7.
Inhibition of Drp1 by P110 reduces loss of neurites in primary dopaminergic neuronal cells exposed to MPP+. Primary rat dopaminergic neurons (cultured for 6 days) were treated without or with P110 (1 µM) followed by treatment with or without MPP+ (1 µM). (A) At 2 hours following MPP+ treatment, the cells were stained with MitoSOXTM (to measure mitochondrial superoxide production) and anti-TH antibodies (a marker of dopaminergic neurons). (B) At 15 hours after MPP+ treatment, cells were stained with anti-TH antibody and anti-Tom20 antibody (a marker of mitochondria). Right-hand panels are enlarged images of boxed areas in each left-hand panel. Scale bar: 0.5 µm. Quantification of mitochondrial superoxide production (C), mitochondrial fragmentation (D) and neurite loss (E) are provided in histograms as means ± s.e. of three independent experiments. Con, control.