Fig. 1.

Structure of Sly gene. (A) Schematic diagram of the structure of the Sly gene (GeneID: 100040308) and of its alternative splice variants: Sly1 and Sly2. The boxes numbered 1 to 10 represent exons, with the coding region in gray and alternatively spliced exons in hatched gray. Short hairpin RNAs (sh344 and sh367) were designed to target sequences located in exons 5 and 6 (indicated in black beneath the schematic of the Sly gene). Because exons 3–4 share ∼86% identity with exons 5–6, sh344 and sh367 can partially recognize sequences in exons 3–4 (indicated in grey beneath the schematic of the Sly gene). (B) Alignment of sh344 and sh367 sequences with Sly1 and with Sly2 cDNA sequence. (C) Alignment of the amino acid sequence derived from exons 3–4 and exons 5–6 of Sly gene.