Figure 5.

SPLIFF analysis of the interactions between components of the polar cortical domain. (A) Selected frames of the time-lapse analysis of a cell (white frame) expressing Spa2CCG and N_ub-Pea2p after mating. The haploid cells are not framed. PCDI indicates the stained region below the membrane of the shmoo tip, PCDII the region below the membrane of the growing bud, and PCDIII the region of cell separation. (B) Time-dependent relative fluorescence intensity (RFI) of Cherry (), GFP (), and the calculated fraction of converted Spa2CC () of the experiment shown in (A). Time 0 indicates the time point shortly before the fusion of the cells. (C, D) As (A) and (B) but with diploid cells expressing Spa2CCG and N_ub-Ptc1p after mating. The cytosolic protein N_ub-Ptc1p does not interact with Spa2CCG. (E) Time-dependent change of converted Spa2CC through interaction with N_ub-Spa2p (), N_ub-Pea2p (), and N_ub-Pea2p in cells lacking the native Pea2p (), N_ub-Hof1p (), N_ub-Hof1_98–669 (), and N_ub-Ptc1p (). Dashed lines separate the analyses of the subsequent PCDs. The averages of n=6 independent matings (error bars, s.e.) are shown. Scale bar, 5 μm. See also Supplementary Figure 3; Supplementary Movies 5–8. Source data for this figure is available on the online supplementary information page.

Source data for Figure 5B ^{(28KB, xls)}

Source data for Figure 5D ^{(27KB, xls)}

Source data for Figure 5E ^{(62.9KB, xlsx)}