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Effect of MG132 on the levels of truncated forms of Borealin. HeLaM cells were transfected with Flag-tagged full-length Borealin, Bor-141, Bor-168, Bor-191 or Bor-221. The cells were blocked in interphase with hydroxyurea and treated with MG132 for 14 h. The lysates were analysed by western blotting with an antibody that recognizes the Flag-tag. Blots were re-probed with an antibody to β-actin to serve as a loading control.
