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Highly aggregated forms of intracellular mutants detected by density-gradient ultra-centrifugation. (A) Total yeast cell lysate containing rFXIII-As (T) was subjected to sucrose density-gradient ultra-centrifugation, and each fraction was analyzed by western blotting. (B) The intensities of rFXIII-A’s bands were quantified by densitometry, and results for each rFXIII-A are shown as percentage of total cell lysate. WT, circle; R260C, square; Del-IV, triangle.
