Fig. 7.

Lost enzymatic activity of mutants. (A) TGase activity of rFXIII-As measured by an amine incorporation assay. TGase activity of each yeast cell lysate was determined by employing monodansylcadaverin and dimethylcasein as substrates. The results represent mean values and SD of three parallel measurements. (B) Fibrin cross-linking activity of rFXIII-As. The indicated amounts of yeast cell lysates for rFXIII-As were incubated with 0.5 mg/ml of fibrinogen, 10 mM CaCl₂, and 20 units/ml of thrombin for 5, 15 and 60 min at room temperature.