Fig. 2.
Effect of adoptive transfer of splenic dendritic cells into SJL mice. YFAK-treated splenic dendritic cells have a modest suppressive effect on EAE. YFAK (150 µg day−1) was injected for 10 days daily and the spleens isolated 1h after the final injections. After enrichment with CD11b and CD11c magnetic beads, subtypes of myeloid cells were isolated by using a MoFlo cell sorter. Cells (1×106 per mouse) were transferred via tail vain injection followed by PLP139–151 (75 µg per mice) injection at day 1 and pertussis toxin injection on day 2. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.