Fig. 3.

CCL22 produced by dendritic cells and induced by YFAK stimulation. (A) Representative scatter plot of total spleen and enriched myeloid populations are shown. (B) Spleen cells were enriched with CD11b and CD11c magnetic beads followed by further isolation by MoFlo sorting. Naive splenic myeloid cell subpopulation G1:CD11b⁺ CD11c⁻, G2:CD11b⁺ CD11c⁺, G3:CD11b⁻CD11c⁺ secreted large amounts of CCL22, and splenic B cells secrete CCL22 in small amounts. Splenic T cells did not secrete any CCL22. Each cell population (1×10⁶ cells ml⁻¹) was stimulated with different doses of YFAK for 72h. CCL22 in supernatant was analyzed by ELISA. (C) Pretreatment of mice with YFAK enhanced CCL22 production from myeloid cell subpopulations. YFAK (150 µg day⁻¹) or vehicle (PBS) was injected s.c. into SJL mice daily for 10 days. Spleen cell populations were isolated 1h after the final injections and analyzed as described in (B). (D) BMMϕ and BMDC were produced as described in Methods and assayed for CCL22 production as described in (B). NT, not tested; ND, not detected.