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Luciferase assay of HEK293T cell transfectants. Mock- or hTLR1/2-, hTLR2/6-, hTLR2/10-, hTLR4/MD2/CD14- or hTLR5-transfected HEK293T cells were cotransfected with an NF-κB luciferase reporter vector were stimulated with YFAK (10 µg ml−1), YEAK (10 µg ml−1) or positive controls (Pam2CSK4 100ng ml−1, Pam3CSK4 100ng ml−1, LPS 100ng ml−1 or rec FLA-ST 100ng ml−1).
