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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: J Am Chem Soc. 2013 Feb 18;135(8):2979–2982. doi: 10.1021/ja400448f

Figure 1.

Figure 1

A genetic AND gate for proteomic labeling. Two promoters (P1 and P2) are used as inputs to drive expression of E. coli MetRS fragments F1 and F2. tRNAMet is charged with Anl only when the MetRS fragments are both expressed, and associate to form a functional enzyme. Ribosomes use Anl-charged tRNAMet during protein synthesis in competition with Met-charged tRNAMet. Newly synthesized proteins are labeled with azide groups, which can be selectively tagged by the azide-alkyne cycloaddition4. Diverse promoter inputs can be plugged into the AND gate to control proteomic labeling with Anl. The MetRS image was created using PDB 2CSX5.